FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717377
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214160.5761051408022377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54370.1462590423301161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA35600.022.02761367
TAGTGGG1003.9343594E-421.00122659
GGGGAAA38850.020.1848968
GGGGGGA43350.019.30092666
GGGAAAA45050.017.5628469
TTAGTAT2701.1691125E-512.96023311
TAGGGTT7550.012.51476222
GGAAAAA68500.012.47039870
GATTAGT2559.835449E-512.3515029
TCGGTGG4306.6411303E-912.20985251
TAGATCT4902.8921932E-1012.14340345
CTAATTA2701.6732395E-411.6671923
AGTGTAC4802.7102942E-911.66703429
TACCCTA2150.004787408311.39294916
GGTCGCC6209.094947E-1211.29067956
GTCCTGG5107.01948E-910.97970436
CTCGACG5804.129106E-1010.85978117
GAGTGTA5209.513315E-910.76797728
GGTGGTC5006.1474566E-810.50075553
TCTCGAC7101.2732926E-1110.34993316
Produced by FastQC (version 0.11.2)