FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415905
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG98500.22305733479320772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82140.18600943634430542No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC73750.16700993341115808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAG15450.045.75337220
CTAGAGT15500.045.1542421
CAACTAG15300.044.14393618
AGGTAAC17150.041.82878512
AACTAGA16250.041.56321319
CAATAGA17000.039.73494339
GGTAACA18000.039.27075613
CCAATAG17400.038.21761738
TCGCTTA15500.037.4848152
ATTAGAG20450.035.599117
GTACAAT20000.035.5222726
AGAGGTA21200.034.33348510
TAGAGGT21550.033.777789
ACAACTA21050.033.08197417
GAGTACA22050.031.58487724
TAGAATC21050.031.4256142
GAGGTAA23750.030.94148811
ATAGAAT22250.030.51727541
ACATCGC22800.029.16772749
CACATCG22900.028.88751648
Produced by FastQC (version 0.11.2)