FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126017
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1269603.077059546773559No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG99470.24107995677186983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9500.039.791816
TCTAGTT10950.035.161887
CTAGTTG11300.032.2095958
ACTCTAG11700.032.0104265
GGAAAAA190200.030.92188870
GGGGAAA189850.029.4288868
GGGAAAA195100.029.14043669
GGGGGAA192900.028.94542367
TGTACTC13750.028.2586022
TGTTACC14850.023.798913
ACCTCTA15000.023.32791717
TACTCTA18150.023.1418554
GTACTCT17700.023.1369533
TACCTCT15900.022.44761816
TAGTTGT17750.022.0825929
GGGGGGA266700.021.61832666
TTGTACT18450.021.064021
GTTACCT16900.020.70500214
TTACCTC18400.019.77777715
GGGGTAA18050.019.0064267
Produced by FastQC (version 0.11.2)