FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458579
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1345963.018809356075108No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC57150.1281798528185774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT10100.039.14940321
GAGTGTA9450.032.9583328
AGTGTAC9250.032.54429
ACTAGAG12100.032.10004820
TCGGTGG8950.032.0686551
TAGATCT9000.031.50016845
CAATAGA12100.031.23843639
CCAATAG11800.031.14283438
GGAAAAA186350.030.60577270
GGGAAAA179950.030.33234269
GGGGAAA178000.030.3278768
CGGTGGT10000.030.10252452
GGTCGCC10500.030.0008356
GGTGGTC10400.029.95409853
GGGGGAA181750.029.58688467
GTGTAGA10950.029.4058942
TAGAGTA13100.029.38419322
GTAGGGA10750.029.29557819
AACTAGA13300.029.20380219
CAACTAG13500.028.7711518
Produced by FastQC (version 0.11.2)