FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301804
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG737152.2325674086045084No Hit
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG139420.422254016289277No Hit
AAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAA75030.2272394121516601No Hit
GGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTG71070.21524596856748612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG61940.18759441808175167No Hit
AAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAA51600.1562782042786307No Hit
GGCAATTTCAAAATCAGGGATAGGACCGTCCACTAGACAAGCGGCTCCGA47180.14289158290437592No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC46870.14195270221975623No Hit
TAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGTCCACTA46680.14137725921950545No Hit
GAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGCTCGATCG42500.12871751321398847No Hit
ACCGAATAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGT34200.10357974004513897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA107700.029.84135468
GGGGGAA110850.028.6143667
GGGAAAA117850.027.68754869
GGAAAAA122900.027.51841470
CCAATAG5600.027.49990538
CTAGAGT6200.024.83185421
CCGTGTA7150.023.98629440
GTAGGGA8750.022.79370519
ACTAGAG7050.022.33425920
GTCGTGT8950.022.28401214
GGTCGCC5500.022.27366356
TGTAGGG9050.022.03811518
CGTGATA9750.021.9026682
CTAGGTC800.002670829721.871288
GGTGGTC5300.021.79435753
ACGGTCC16400.021.77481569
AGAGCCG9100.021.53642836
GGGGGGA152650.021.32903166
AGGGAGG48300.021.29814314
AAGGGAG48800.021.15162713
Produced by FastQC (version 0.11.2)