FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956712
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG876392.214945136264656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG78350.1980179502576887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG8750.040.003325
CTCTAGT9800.036.073976
TCTAGTT10450.033.8292777
TACTCTA11100.032.7955174
GTACTCT10850.032.5853
CTAGTTG10800.032.4047748
GGGGAAA128400.030.4799268
GGGGGAA131300.030.09998567
GGGAAAA133050.029.94124869
GGAAAAA142450.028.9238870
TGTTACC12050.027.58658613
TGTACTC13950.025.848362
GTTACCT13100.024.30700714
TAGTTGT16200.022.8993729
TACCTCT14050.022.4147116
TTACCTC14100.022.33493815
GGGGGGA179650.022.19389766
ACCTCTA14700.021.42358217
TGGACAC15850.019.43035128
TTGTACT18350.019.271021
Produced by FastQC (version 0.11.2)