FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t0_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t0_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4103057
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101720.24791271483676683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG6000.026.8379945
CTCTAGT7700.022.276056
GGGGGAA36150.021.11196767
TACTCTA8000.021.0036474
CACTAGT6400.020.23246236
GGGGGGA40650.019.54995766
CCACTAG6450.019.53708535
GTTACTA6950.018.636333
TCTAGTT11100.018.2905717
ACTACCC7150.018.1145926
GGGGAAA42950.017.85095668
GTACTCT9900.017.326243
ACTAGTA7550.017.15132337
CTAGTAG7650.016.46963338
TACCCTA8200.016.2177818
TACCTCT10150.016.20240216
TACTACC8000.016.1903115
TACACTC8300.016.01965313
CTACCCT8750.015.2018987
GTTACCT10900.015.08755914
Produced by FastQC (version 0.11.2)