FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477288
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG977412.8108399419317585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG54170.15578232231555167No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC49050.14105820397965313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38890.11184003165685442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT8200.049.07303221
CCAATAG9950.039.74675838
ACTAGAG10750.037.43245320
TAGATCT7950.036.54326245
TAGAGTA11150.036.40393422
TCGGTGG7900.035.88789751
AGAGGTA11800.035.88081410
GGTGGTC8650.035.60976853
TAGAGGT11700.035.592429
AACTAGA11300.035.30086519
AGAGTAC11550.035.1431923
CGGTGGT8850.033.61849252
GGTCGCC9200.033.48044256
CAACTAG11950.033.38073318
ATTAGAG13050.032.1860057
GAGTGTC10300.031.9355628
GTGGTCG9500.031.68673154
GTGTAGA10100.031.53534742
GAGGTAA13950.030.35079611
CAATAGA13150.030.3406939
Produced by FastQC (version 0.11.2)