FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860492
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91570.32011975562245937No Hit
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG76920.2689047898053901No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC32610.11400136759690291No Hit
GTGAACTACTGTGGAAGTTACTGACGTAAGATTACGCCGCGTGAATACCG31840.11130952297716618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG500.00720620328.00472368
GCTAGAT3550.025.6448541
AAGGGAG24250.023.0873913
CTAACGT6850.021.9802721
TACCCCC11850.021.85220111
CCTAGGT5350.021.5980911
AGGTGGT7350.021.4306876
TTACCCC12100.021.4007110
CTTACCC12650.021.0260669
ACCCCCA12650.020.46988712
GGAGGAT28450.019.92504916
CACACTA4600.019.78352462
GGTGGTG8150.019.755867
CACTAGG4300.019.53578664
TATCGAC9350.019.4671615
TGGGTAC4500.019.44432437
GTGGTGA8300.019.3961128
AAGGTGG8050.019.1323225
AGTATAC8450.019.05480231
GGGTAAT5450.018.6318821
Produced by FastQC (version 0.11.2)