FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666807
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2574945.51756265043744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG219380.4700858638465229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG61960.13276743606495833No Hit
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG57990.12426054902206156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA331800.035.37994870
GGGAAAA357550.031.5586869
ACTCTAG6650.031.0570745
GGGGAAA363950.030.44448968
GGGGGAA379450.029.15474367
TACTCTA7600.027.1746464
CTCTAGT7300.026.3736346
CTAGTTG7850.026.3021648
TCTAGTT8050.024.7848247
GTACTCT8850.023.3364223
GGGGGGA525350.021.49128366
GAAAAAA244200.020.2006570
GGGGTAA30000.019.60490467
TCGGTGG4650.018.06516351
ACCTCTA10700.017.65869717
GGGTAAA34650.017.4791268
TACCTCT10900.017.01367216
TAGTTGT13400.016.975319
GGGGGTA34200.016.78800666
GTTACCT10750.016.60008614
Produced by FastQC (version 0.11.2)