FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t-1_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t-1_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096000
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG642801.5693359375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG73410.1792236328125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG52140.12729492187500002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG50840.12412109375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG49120.119921875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG4700.040.961225
GGAAAAA219050.032.09322470
GGGGAAA217300.029.90129368
GGGAAAA223700.029.87671769
ATAGGGT5450.029.54003537
GGGGGAA222150.029.1696967
GGATAGG5750.028.0022435
CTCTAGT7200.027.7105486
TCGGTGG5750.027.3924951
TACTCTA7350.026.1928884
GATAGGG6750.024.36874636
GTACTCT9100.023.4639853
GGGGGGA281700.023.36375866
TCTAGTT10050.023.3343417
GTTACTA8850.021.358163
ACTACCC8600.020.7574716
TACCCTA9100.019.9969568
GGGGTAA21200.019.98118467
GGTCGCC8600.019.94272456
GTGGTCG7750.019.87279954
Produced by FastQC (version 0.11.2)