FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_659t0_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_659t0_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734434
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83900.4837312921679349No Hit
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA23370.13474136231185505No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA22740.13110905344337115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG2700.046.666555
TACTCTA3550.037.46474
CTCTAGT3800.034.0788656
GTTACCT3800.033.15016614
TCTAGTT4900.030.714217
GTTACTA2800.028.749933
GTACTCT4950.026.868623
ACCTCTA4650.026.33870517
GGGGGAA27600.025.49421367
TACCTCT4850.025.25257516
GGGGAAA28100.025.1651668
ACTACCC3250.024.7691696
TGTTACC5350.024.19996513
GGGGGGA30150.024.03464366
TACTACC3350.024.029795
TTACCTC5150.023.78086515
GGACACC5400.023.33394829
TGGACAC5850.022.7330228
CTAGTTG5850.022.733028
TTGTACT6000.022.1730061
Produced by FastQC (version 0.11.2)