FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_659t-1_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_659t-1_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529469
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301610.6658837934424543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG5300.028.3972535
GGGGGAA71050.025.86614667
TACTCTA6750.024.3712524
GGGGAAA74100.024.09286568
GGGGGGA80500.023.91681566
ACTACCC7900.021.7091646
GGGAAAA85550.021.23654469
CTCTAGT7550.020.861226
GTTACTA7500.020.5338483
TACTACC7900.020.3804825
TTACTAC9000.017.8895344
CTACCCT9250.017.7840357
TCTAGTT10550.017.5831837
GGAAAAA108200.017.53549870
TACCCTA9200.017.49878
CACTAGT8500.016.88165936
GTACTCT9550.016.8590623
TACACTC9450.016.6635913
CCACTAG8050.016.52215435
CCCTACC4300.016.28163566
Produced by FastQC (version 0.11.2)