FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_wt0_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_wt0_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5121067
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3626707.081922575900686TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT129290.25246691753886447TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG91540.17875181090190775TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT59160.11552280022893667TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG412750.065.4597541
TCTCGTA404700.065.33526643
AGTCACG462450.064.7960228
GAACTCC463050.064.7668921
GTATGCC412900.064.7249447
CTCGTAT408100.064.6537244
TCGTATG402950.064.5420145
CGTATGC408500.064.4104946
GTATCTC422000.064.3146240
ATCTCGT415750.064.2890342
CGTATCT433550.064.2645839
ACTCCAG466750.064.2459723
CACGTCT467800.064.2289314
TGAACTC467300.064.2003220
CACACGT468750.064.1877312
CCAGTCA467200.064.1372726
GTCACGA467200.064.1297529
ATTCGTA463400.064.1079936
ACACGTC469700.064.0510713
GCACACG469700.064.04311
Produced by FastQC (version 0.11.2)