FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_wt-1_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_wt-1_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653563
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT36358755.6315152479562TruSeq Adapter, Index 2 (97% over 37bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG89641.37155867146702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51950.7948736388075824No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA46430.710413533201849No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG43180.6606861159520965TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA42860.655789877945967TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.6487515358121558No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA35900.5492967013126508No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA35880.5489906864372677No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA31310.4790662874122311No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT29040.44433359905625014No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA26780.40975391813796064No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT24450.3741031851558304No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT22130.3386054596113917No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA20800.31825547039841606No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCT20500.3136652472676697No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATC20490.3135122398299781No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC19850.3037197638177192No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA16700.2555224209448821No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC16090.24618896724569783No Hit
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA15600.23869160279881207ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA14340.2194126656496772ABI Solid3 Adapter B (100% over 41bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG14060.21512845739431394No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT13700.20962018963741827TruSeq Adapter, Index 2 (97% over 35bp)
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC13410.2051829739443634No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT13340.20411192188052263No Hit
GCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA13300.20349989212975644No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.2027348549412987No Hit
CCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTA12570.19233034917827355No Hit
GATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCT12170.18621005167061172No Hit
CTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAA11860.18146682110217377No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT11730.17947772441218368TruSeq Adapter, Index 2 (97% over 37bp)
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT11310.17305141202913873No Hit
TAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATT11200.17136833021453174No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG11050.16907321864915853No Hit
CTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA11010.16846118889839234No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT10920.16708412195916844No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT10840.16586006245763604No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT10280.15729164594690948No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT10190.15591457900768554No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTAC10040.15361946744231236No Hit
CTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGAT10000.1530074376915462No Hit
CACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTA9480.1450510509315858No Hit
GCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGT9450.14459202861851114No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT9450.14459202861851114No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC9390.14367398399236186No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG9250.14153187986468022No Hit
TAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAA9240.14137887242698868No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA8970.13724767160931692No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA8940.1367886492962423No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.1318924112901128No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT8450.12929128484935654No Hit
GGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG8320.12730218815936642No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG8180.1251600840316848No Hit
AGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAAC8110.12408903196784396No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT8100.1239360245301524No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.12301797990400312No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA7520.11506159314404274No Hit
CTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTC7480.11444956339327654No Hit
GGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGA7460.11414354851789345No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT7390.11307249645405262TruSeq Adapter, Index 2 (97% over 37bp)
TGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACA7300.11169542951482872No Hit
CCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGG7200.11016535513791326No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT7060.10802325101023161TruSeq Adapter, Index 2 (97% over 35bp)
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC6980.10679919150869924No Hit
CTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTA6910.10572813944485843No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC6910.10572813944485843TruSeq Adapter, Index 2 (97% over 34bp)
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC6820.1043510725056345No Hit
TATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAAC6780.10373904275486831No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT6780.10373904275486831No Hit
GTTTGGTACTATCTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGG6750.10328002044179367No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG6630.10144393118949513No Hit
CTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGGACTATCAGGTGT6570.10052588656334584No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT6540.1000668642502712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT301.0223448E-770.012437
ATGATCT150.00222291270.012439
ACATTAT600.070.012437
TTATCAC353.7052814E-970.012440
CGTTATC252.8366358E-670.012438
CATAATC301.0223448E-770.012438
TCATAAT301.0223448E-770.012437
TATACTA150.00222291270.012437
CCATTAT150.00222291270.012437
CGCACAT550.070.0070434
GCTCGGA150.002223586770.007041
CGCTAAT401.3460522E-1070.00703434
CATCGGA2000.070.0070341
ACACGTA301.023418E-770.00168613
GCAAGAG150.002224261670.0016865
TCACCAC207.925117E-570.00168630
ACCTCTC207.925117E-570.00168632
CCGACTG2000.070.0016865
TGGTATG150.002224261670.00168645
AATCCAG301.0239637E-769.9963323
Produced by FastQC (version 0.11.2)