FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472199
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT36020.10373829380170894TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG15350.049.70566632
CTCGCGC16050.047.5467235
TCTCGCG16250.046.7413934
CTCTCGC16950.045.2201733
CACACGT17350.044.78280312
CGCGCAT17300.043.09953337
CTCCAGT18150.042.8101424
TCCAGTC18400.042.79913325
GCACACG18450.042.3037411
TCGCGCA18200.041.92993536
ACTCCAG18900.041.48169723
GTCACTC18350.041.3880729
CCAGTCA19250.040.54566226
CAGTCAC19500.040.20417827
AGTCACT19600.039.82048428
GAACTCC19900.039.2218721
CACGTCT19900.038.86842714
ACACGTC20450.038.16535613
TCACTCT21600.035.4852730
GCGCATC22250.033.6684338
Produced by FastQC (version 0.11.2)