FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5166092
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT383360.742069634067686TruSeq Adapter, Index 7 (97% over 36bp)
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA68680.13294381904155017No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG66200.12814328509829093No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG57230.11078006353738958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG52150.054.42917623
GTATGCC52400.054.17001747
CACACGT54000.052.95079812
AGTCACC53550.052.9382728
CCGGCTA53150.052.8129633
TCACCGG54000.052.3057230
GCACACG54800.052.24115411
TCCAGTC55150.051.59531425
CCAGTCA55150.051.46838826
CTCCAGT55050.051.43472324
TCGTATG56050.051.0161245
ACACGTC57650.049.5983213
CAGTCAC57550.049.44124627
GGCTATG57050.049.396735
CACGTCT57900.049.3841614
CGTATGC58650.048.3964946
GCTATGA58250.048.37908636
AGCACAC60700.047.6822610
GAACTCC59950.047.4067821
CTATGAT59250.047.3257737
Produced by FastQC (version 0.11.2)