FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432278
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT274230.6187111909496652TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT96710.2181947973480003TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGTAT50710.11441069355306684TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGTAT49470.11161303510294254TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC93650.061.4546935
CTCTCGC94200.061.27063833
ACTCTCG94350.061.2103232
GTCACTC95550.060.765129
TCACTCT95650.060.63523530
ACTCCAG96450.060.45958723
TCTCGCG96300.059.90154334
AGTCACT97400.059.7187328
CGCGCAT96550.058.92057437
CCAGTCA98900.058.89040426
CAGTCAC99600.058.504427
CTCCAGT100000.058.3132724
CACTCTC99900.057.80974631
GAACTCC101900.057.3640221
CACACGT102400.057.28255512
TCGCGCA100350.057.17716236
TCCAGTC102850.056.86691325
GCACACG103400.056.62637711
TGAACTC103100.056.5605520
GTATGCC47050.056.1636447
Produced by FastQC (version 0.11.2)