FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3848064
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1652344.293951451950903TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.1475547184246416No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA41380.1075345940192263TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG194350.064.7547528
GTATGCC194900.064.1427347
ACTCCAG199600.063.11069523
CGTATGC198950.062.87216646
GTCACGA200200.062.84508529
TGAACTC200250.062.8359320
TCGTATG198800.062.7963745
CAGTCAC200900.062.695827
GCACACG202000.062.66705711
CATCTCG194700.062.66510441
GAACTCC202200.062.28187621
CACACGT203400.062.1496912
CACGAGA202200.062.144231
CCAGTCA202550.062.120826
CGAGATT201850.062.11404433
CTCCAGT203100.061.98864724
TCTGAAC204050.061.7834118
TCACGAG204050.061.66574530
ACGTCTG204800.061.60681515
CTCGTAT201300.061.56444544
Produced by FastQC (version 0.11.2)