FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220293
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1340546.037671604603537TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG153900.065.197728
CATCTCG149150.064.7133141
GTATGCC155150.064.38061547
CAGTCAC156200.064.2376827
CACACGT158200.063.93583312
GAACTCC157200.063.8556221
ACACGTC158300.063.80700313
TCCAGTC157150.063.7868525
GCACACG158650.063.75448211
CTCCAGT157350.063.72801624
CCAGTCA157300.063.7037726
TCGTATG156700.063.6306945
CGAGATT157700.063.56437733
CACGTCT159000.063.50408614
GCCGTCT155000.063.49456851
GTCACGA158150.063.4677529
ACTCCAG158300.063.34556623
TGAACTC159000.063.22077620
CGTATGC157900.063.21360846
CCATCTC152900.063.1490640
Produced by FastQC (version 0.11.2)