FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027105
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1183842.939679993444422TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG133200.064.5210541
GTCACCT136950.064.14429529
GTATGCC136250.063.83404547
ACTCCAG137800.063.77798523
AGTCACC139500.063.04780628
CTATCTC136100.063.0433640
CACACGT140800.063.037412
CCAGTCA139700.062.95989226
GAAGCTA139250.062.64875436
CGTATGC139250.062.53343246
TGAACTC141050.062.53100220
GCACACG142000.062.5285611
CAGTCAC141450.062.30235727
TCGTATG140050.062.20121445
AGCTATC139650.061.59192338
CTCCAGT143500.061.31781424
GAACTCC143950.061.14968521
AGAGCAC145550.061.1477478
AGCACAC146850.060.5587710
AACTCCA145450.060.49574722
Produced by FastQC (version 0.11.2)