FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4928912
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3189456.470900677472026TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT119050.24153403428586268TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA62880.12757379316165515TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT54530.11063293481401168TruSeq Adapter, Index 7 (97% over 35bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC50860.10318707252229295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG360050.065.4506641
AGTCACG411650.064.7001928
CGTATCT378300.064.47761539
GTATGCC364950.064.3887947
GAACTCC414700.064.3094321
ATTCGTA411100.064.2372536
GTATCTC369250.064.2092740
GCACACG418550.064.08446511
GTCACGA416150.064.0167329
ACACGTC419950.063.88749713
ACTCCAG417300.063.8764923
TCGTATG354450.063.8671145
CACACGT420350.063.826712
CTCCAGT417250.063.81703624
TGAACTC419300.063.71243720
CAGTCAC418150.063.7111927
TCCAGTC418650.063.6428425
CCAGTCA418450.063.63980526
AACTCCA419700.063.56960322
CGTATGC363450.063.52783246
Produced by FastQC (version 0.11.2)