FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT83960.20991931257675706TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG15150.039.047941
GCACACG15850.037.7589411
CACACGT16250.037.2602512
ACACGTC16600.036.2642613
CACGTCT18450.032.817714
AGTCACC18200.031.92196828
ACCGCTC18350.031.8533532
CAGTCAC18900.031.2952127
CCAGTCA19400.030.30936626
AGCACAC20300.029.8265510
CTCCAGT20200.029.62916824
TCCAGTC20550.029.1241725
ACTCCAG21050.028.9315623
AGAGCAC21250.028.4927758
GTATGCC20950.028.4006847
GAACTCC21000.028.33412421
TCGTATG21000.028.33306145
CGTATGC20900.028.30116346
TGAACTC22000.027.04620720
CACCGCT22350.026.4657331
Produced by FastQC (version 0.11.2)