FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_vgt0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_vgt0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190830
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT574621.800848055208206TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC69000.064.6737547
TATCTCG69200.063.940541
ACTCCAG71250.063.17276423
CACACGT71600.062.7632412
GCACACG72250.062.3439111
TGAACTC72550.062.13922520
TCGTATG72450.061.6906745
AGTCACC72700.061.62102528
CGTATGC73000.061.0820446
CAGTCAC73550.061.0040527
ACCGCTC73200.060.8185732
CCAGTCA74050.060.6413126
CACCGCT74000.060.39755631
AGAGCAC75950.059.306758
ATTATCT74750.059.1453239
GAACTCC76550.058.8465221
AACTCCA76650.058.81448422
TCTGAAC77350.058.55372218
CTCATTA76050.058.50251836
CTCCAGT77200.058.39455824
Produced by FastQC (version 0.11.2)