FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_sigg45_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_sigg45_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398164
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT391809.840166363608965TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT103982.611486724063451TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGTT77651.9502014245386323TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGAT72541.8218623481781375TruSeq Adapter, Index 6 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72271.8150812228127102No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTAT61201.5370550828301905TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGAT61141.5355481660823178TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTAT52631.3218171406757013TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGAT41091.031986819501512TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGGT40561.0186757215619695TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.8938527842798446No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTTT33890.8511568097567836TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTTT33240.8348318783214956TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTTT24930.6261239087411217TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTTT24360.6118081996363308TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGTT22200.5575591967129123TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGGAT22120.5555499743824153TruSeq Adapter, Index 6 (97% over 36bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC16240.4078721330908872No Hit
CCTTGTAAGCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTAC12560.31544790588802607No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGGTT12080.30339257190504415TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGGT11920.2993741272440502TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGTT10890.27350538973890154TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGT10690.26848233391265913TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA10560.2652173476256015TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGTT10100.25366431922524385TruSeq Adapter, Index 6 (97% over 36bp)
GCCTTGTAAGCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTA8270.2077033584151254No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT7060.17731387066635856No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGGT6030.15144513316120994TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAA5240.13160406264755226TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTT5030.1263298540299977TruSeq Adapter, Index 6 (97% over 36bp)
TGCCTTGTAAGCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTT5030.1263298540299977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA43900.066.0103543
CTCGTAT43650.065.8271644
GGGTTCT3750.065.3300554
GTATGCC50800.065.2427847
ATCTCGT48950.065.2061242
GTGGGTG14450.065.1606259
TCGTATG41500.065.1051645
AATCTCG54800.065.0951941
CGTATGC45100.064.7971946
CTGCGTG16050.064.7712459
AAATCTC56450.064.5568140
TATGCCG50150.064.55308548
GCCGTCT37100.064.5250651
AAAAAAG124500.064.5148370
ATGCCGT42000.064.4134349
TCTGCTT39200.064.2905658
GAAATCT72550.064.223839
GTGGTTG6050.064.2197259
TGGGTGG6650.064.2153660
CTTCTGC42100.064.0941756
Produced by FastQC (version 0.11.2)