FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_sige45_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_sige45_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571536
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT122402.1415973796926178TruSeq Adapter, Index 7 (97% over 35bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG6190.10830463872791914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG15450.063.42986728
TATCTCG14200.062.36406741
ATTCGTA15450.062.30264336
GTATGCC14350.062.1945847
ACTCCAG15950.061.660923
CAGTCAC16100.060.86903827
GCACACG16200.060.70934311
ACACGTC16350.060.3664413
CACACGT16300.060.33689512
GAACTCC16350.060.15237421
TGAACTC16350.060.15237420
TTCGTAT16100.060.0047337
TCCAGTC16350.059.9383125
TCGTATC16300.059.48321538
CTCCAGT16550.059.4254624
CGTCTGA16600.059.2464716
GAATTCG16350.058.86798534
CGAATTC16550.058.79102733
AACTCCA16700.058.68212522
GTCACGA17000.058.05831529
Produced by FastQC (version 0.11.2)