FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_sige35_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_sige35_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041834
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2316073.8333890007570552TruSeq Adapter, Index 7 (97% over 36bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG194970.32270002783922896No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC146310.2421615688216525No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA138970.2300129397795438No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG128570.2127996234256022No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA98410.16288100599917177No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC92860.15369505352182797No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC92140.15250336238963202No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC88900.14714075229475024No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT76220.1261537473555215No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG73530.12170145687551163No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT70550.11676917968947839No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT62920.1041405639413463No Hit
GAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCATT61200.10129374623665595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG263100.064.681823
GTATGCC262750.064.6709747
CACACGT267200.064.1545212
GCACACG267400.064.1332411
GGCTATG265450.063.997435
CCAGTCA266150.063.8090626
AGTCACC266300.063.8067428
CCGGCTA267400.063.56102833
CTATGAT263850.063.55017537
TCCAGTC267600.063.50254425
CTCGTAT265650.063.45009644
CGTATGC269300.063.2139446
TGAACTC270400.063.16858320
GCTATGA267500.063.1667136
ACACGTC271850.063.07002613
AACTCCA270600.063.0184222
TCGTATG270350.062.92959245
CTCCAGT270750.062.841324
CAGTCAC270850.062.81237827
GAACTCC271950.062.74419821
Produced by FastQC (version 0.11.2)