FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_s87745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_s87745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497328
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT182610.7312215295708053TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG24250.052.83923741
ACTCCAG25150.051.6330123
GTCACCT25550.050.2706529
AGCTATC25600.050.18854538
AGTCACC25750.049.881228
GAACTCC26100.049.62053321
CACACGT26550.049.0363112
GAAGCTA26700.048.64630536
GCACACG26900.048.39828511
CTCCAGT26700.048.2423124
GTATGCC27150.047.69577847
ACACGTC27400.047.5151113
CAGTCAC27200.047.4794327
TGAACTC27600.047.17740620
AACTCCA27750.047.17466422
CTATCTC28000.046.26176540
TCCAGTC28200.045.67622825
AAGCTAT28300.045.6476537
TCGTATG28400.045.59649345
AGCACAC28600.045.5214710
Produced by FastQC (version 0.11.2)