FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780873
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT757632.003849375527821TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT201810.5337656144493613TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGTT140680.3720833786271054TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGAT134460.35563215162212536TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGAT120570.31889460450007184TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTAT112690.2980528571046951TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTAT98480.2604689446061796TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGAT81980.21682822988235784TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTTT62880.16631079647478242TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTTT60430.15983081156124523TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTTT45900.12140053368626769TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGGAT43690.11555532280507702TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTTT43220.11431222365839847TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGTT42660.11283108424958999TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC271250.065.601721
ACTCCGC268550.065.4783932
CTCCGCG269150.065.3454333
GTCACTC271600.064.95872529
TGAACTC274550.064.9279220
CACTCCG271650.064.7827131
TCCAGTC274100.064.5348125
AGTCACT274400.064.4863528
CTCCAGT275400.064.2954224
CACGTCT278200.064.1850614
GCACACG278900.064.0992511
ACGTCTG278350.064.0750315
CACACGT279150.064.0418412
TCACTCC275300.063.94923430
ACTCCAG277450.063.9465123
ACACGTC280150.063.82573713
AACTCCA278650.063.79673422
CCAGTCA277500.063.75671426
CAGTCAC277950.063.7130927
CTGAACT281600.063.36121719
Produced by FastQC (version 0.11.2)