FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021358
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT36449812.064045372974668TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA53730.17783394089677557TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG42290.1399701723529618TruSeq Adapter, Index 7 (97% over 35bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA36920.12219670757321707No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC33060.10942099545965754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT418550.065.8314612
GCACACG418900.065.78480511
GTATGCC414350.065.763947
CACGTCT419150.065.69546514
ACGTCTG419200.065.6887215
CCGGCTA413300.065.6728233
CTCCAGT416450.065.657324
ACACGTC420150.065.61407513
TGAACTC418700.065.60541520
AGTCACC415050.065.5939628
GAACTCC418200.065.5666821
TCCAGTC416550.065.5659225
TCGTATG415000.065.5428445
GTCTGAA420550.065.4612117
GCCGTCT414650.065.44623651
CCAGTCA417300.065.4396826
GGCTATG415350.065.3627535
TCTGAAC420900.065.3485718
AACTCCA419650.065.3317922
CGTATGC417450.065.3258446
Produced by FastQC (version 0.11.2)