FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864892
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT706281.8274249319256526TruSeq Adapter, Index 13 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39250.10155523103879745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC98350.063.3873221
GCACACG101800.062.0547211
ACGTCTG102050.061.5270515
TGAACTC101900.061.3851320
ACTCCAG101800.061.3758723
GTCACAT102000.061.28111629
GTATGCC87000.060.98611547
AGCACAC104300.060.63442610
CACGTCT103950.060.60368314
ACACGTC104300.060.50098813
AGTCACA104000.060.3045528
AACTCCA104550.059.862722
CTCCAGT104450.059.78519424
CACACGT105750.059.67141712
CACATTC104450.059.61377331
TCCAGTC104700.059.60823425
TCGTATG86500.059.5988245
CTCGTAT87450.059.51167344
CGTATGC89450.058.53320346
TCTGAAC107750.058.24127618
Produced by FastQC (version 0.11.2)