FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723773
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT38230.22178094215421634TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC7550.040.79691721
CTCCAGT7600.040.5285124
ACACGTC8000.038.4987413
TGAACTC8050.038.26294720
CACGTCT8050.038.25961714
CACACGT8250.037.75633612
AGTCACA8750.035.1988528
ACTCCAG8800.035.001923
GCACACG8950.034.80232211
GTCACAT8900.034.60460729
CACATTC9250.033.67457231
TCCAGTC9800.032.50082425
CGTATGC8550.032.33999646
GTATGCC8850.031.64013547
AATCTCG8150.031.35230441
ACGTCTG10000.031.14988515
CCAGTCA10300.030.24348426
GCCGTCT7550.030.1323451
AGCACAC10500.029.99814810
AACTCCA10450.029.81023222
Produced by FastQC (version 0.11.2)