FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649405
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2152095.8970982941054775TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT78360.21471993379742726TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG271900.066.8304728
GAACTCC274300.066.5191421
CTCCAGT274600.066.3062624
GTATGCC243600.066.1043347
ATTCGTA272700.066.0979436
TCCAGTC275500.066.0252225
TCGTATG237150.065.95415545
CTCGTAT241800.065.8727144
TATCTCG248350.065.8418341
CCAGTCA277250.065.6968426
ACTCCAG277500.065.6385523
CACACGT279850.065.63228612
GTCACGA277400.065.51803629
CGTATCT257450.065.5124839
ACGTCTG279450.065.4900715
CGTATGC241450.065.48984546
GTATCTC251400.065.418140
CACGTCT280150.065.4120914
TTCGTAT274800.065.3507937
TGAACTC279450.065.2932620
Produced by FastQC (version 0.11.2)