FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712958
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT619081.0836417841685515TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG434030.759729023038503TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT121690.2130069921746318TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.1044467682065928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT65800.062.92404644
GTCACTC154300.062.64700729
GAACTCC154750.062.6308221
TCGGGGG115800.062.29018443
GCACACG157750.061.56532711
AGTCACT157800.061.3911128
TCCAGTC158350.061.13955725
ACGTCTG159200.061.0056615
CTCCAGT159250.060.79509424
ACACGTC160050.060.74673513
ACTCCGG159500.060.47823332
TGAACTC160400.060.46832320
CTCCGGA159950.060.2424433
ATCGGGG144250.059.8796842
CACTCCG162300.059.52112631
CACGTCT163500.059.44351614
CAGTCAC163750.059.26727327
AACTCCA163550.059.2180922
TCCGGAG162500.059.21301334
ACTCCAG164450.058.89400523
Produced by FastQC (version 0.11.2)