FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175335
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT38310.17611080592184652TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG9600.049.5821143
CGGGGGT5650.049.5574444
ACTCCGG13250.048.07539432
GTCACTC13800.046.411929
ATCGGGG12600.045.55443242
CTCCGGA14500.043.93096533
CACTCCG14800.043.7499331
CTCCAGT14950.043.0788324
GAACTCC15350.041.9562621
CACGTCT15550.041.41377314
TCCGGAG15400.041.1372434
CGGGGGG4900.039.99993544
ACGTCTG16150.039.44175315
ACACGTC16400.039.05391713
AGTCACT16800.038.74904328
CCAGTCA16750.038.65754326
GCACACG16650.038.46840711
CCGGAGA16550.038.28052535
CAGTCAC17550.037.89080427
TGAACTC17400.037.2142920
Produced by FastQC (version 0.11.2)