FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_oat0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_oat0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562640
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT810075.183983515077049TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG565173.6167639379511596TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT168921.08099114319357TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT44910.2873982491168791TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTG31790.20343777197563095TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTGT23280.14897865151282444TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG178700.066.8257142
CACTCCG199500.066.6828431
CGGGGGT86300.066.6345944
TCACTCC199950.066.62028530
TCGGGGG148900.066.6138143
CTCCAGT200900.066.60354624
CTCCGGA199550.066.5784433
TGAACTC201550.066.5624120
GAACTCC201400.066.5424721
GTGGGTG40500.066.5418459
AGTCACT201300.066.48646528
ACTCCAG201450.066.4390823
GTCACTC201200.066.4325329
TCCAGTC201800.066.3390725
ACGTCTG202700.066.3229115
CCAGTCA202000.066.25606526
AATCGGG186900.066.0225641
AACTCCA203150.065.9864822
CAGTCAC203100.065.9661527
CACGTCT204300.065.8699114
Produced by FastQC (version 0.11.2)