FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_csfg35_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_csfg35_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944719
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1213892.04196363192272TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC145500.060.663828
ACTCCAG146250.060.52326623
GTATGCC147050.059.52655447
TATCTCG146800.059.51923441
CACACGT150000.059.28919212
CTCATTA148550.059.03064736
TGAACTC151700.058.60268420
CTCCAGT151400.058.48763324
GAACTCC151600.058.4335621
CCAGTCA151450.058.37588526
CGTATGC149950.058.3514946
TCGTATG150200.058.27767645
AACTCCA152100.058.1954522
CTCGTAT150600.058.05316544
ACACGTC153450.058.04743213
TCCAGTC153300.057.7627425
CAGTCAC153350.057.5812327
GCACACG154900.057.34444411
ATTATCT152350.057.2820639
ACGTCTG155750.057.19022815
Produced by FastQC (version 0.11.2)