FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t45_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t45_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854251
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT562711.4599723785503331TruSeq Adapter, Index 13 (97% over 38bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG42640.1106310927856022No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG41340.10725819361530943No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA40870.10603876083835743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG82750.059.46890623
CACACGT84450.058.76607512
AGTCACA84500.058.275728
GAACTCC85300.057.8552421
CCAGTCA85450.057.67176426
AACTCCA85400.057.66454722
TGAACTC86000.057.5064220
ACACGTC87100.057.0584913
GTATGCC72700.057.0012447
TCCAGTC86950.056.7573625
GCACACG87950.056.6271611
CTCCAGT87650.056.26414524
CAGTCAC87950.055.98972727
CACGTCT89200.055.83289714
AATCTCG74950.055.81315241
GTCACAT88300.055.60925329
ACGTCTG90000.055.2595515
CACATTC89250.055.13783631
CTGAACT89900.055.0881419
TCGTATG71800.054.9852345
Produced by FastQC (version 0.11.2)