FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t35_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t35_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794795
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1531323.193713182732526TruSeq Adapter, Index 27 (97% over 39bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG184490.384771403156965No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA126890.26464113689949204No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC118670.24749754681899855No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG114620.23905088747276995No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC92620.19316779966609626No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA85230.1777552533528545No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC73600.15349978466232653No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC64570.1346668627125873No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT62060.1294320195128259No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG58510.122028157616749No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG53740.11207986994230203No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT51920.10828408722374994No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG48010.10012941116356383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA173150.064.16492536
ACATTAC175800.063.60854332
ACTCGAT173400.063.60812437
GTATGCC176000.063.51703347
ATTACTC177600.062.82918534
CTCGTAT176050.062.7435344
CATTACT178300.062.73629433
AGTCACA179600.062.57254828
TCGATCT175150.062.23314739
CGTATGC179550.062.1832346
TCGTATG179900.062.1011645
TTACTCG179300.062.0616835
ACTCCAG181200.061.910623
CACATTA181500.061.7073431
GTCACAT182150.061.6197129
CCAGTCA183750.061.01014726
CAGTCAC184400.060.81089827
GCCGTCT182700.060.74458351
GAACTCC184850.060.70705821
TATGCCG184250.060.69135348
Produced by FastQC (version 0.11.2)