FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717377
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT277210.7457139805836211TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC37300.054.2455440
GTATGCC38300.053.4582347
TGAACTC39050.053.15208420
AGTCACC39100.052.81130228
GTCACCT39100.052.81130229
TATCTCG38500.052.73804541
GAACTCC39650.052.43604321
GAAGCTA40550.051.4524836
ACTCCAG41450.050.41161723
TCGTATG40950.050.16971645
CACACGT41900.049.86684812
GCACACG42200.049.67754711
CTCCAGT42300.049.31587224
CCAGTCA42350.049.09235426
AGCTATC41900.048.70923238
AAGCTAT42350.048.68763437
TCCAGTC43100.048.4817125
CGTATGC42850.048.02683346
GCTATCT43100.047.1899839
CAGTCAC44950.046.7161827
Produced by FastQC (version 0.11.2)