FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415905
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT458901.0391980805746501TruSeq Adapter, Index 19 (97% over 38bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG103440.23424416965491784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC57500.059.11003540
TATCTCG58350.058.31093241
GTATGCC59150.058.16666847
AGTCACC59900.057.9025528
GTCACCT60300.057.57649229
ACTCCAG60550.057.16860223
AGCTATC61000.055.94800638
CCAGTCA62500.055.66431826
CAGTCAC62900.055.36283527
GAAGCTA63850.054.16339536
AAGCTAT63550.054.08860437
TCGTATG63950.054.01968445
CACACGT64900.053.7664212
GAACTCC64650.053.70547521
TGAACTC65450.053.15598320
GCACACG66150.052.90855411
CTCCAGT65750.052.80697624
CGTATGC66650.051.7263146
TCCAGTC67850.051.1719925
GCTATCT67000.050.8854939
Produced by FastQC (version 0.11.2)