FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126017
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2189595.306788605088151TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC253100.066.0033147
CTATGAT249750.065.792737
CCGGCTA253950.065.50754533
AGTCACC255100.065.35840628
ACTCCAG256700.065.2997923
CCAGTCA256300.065.18082426
TCGTATG256450.065.0993845
CGTATGC257150.065.0310646
CTCCAGT258050.064.8767824
GGCTATG257050.064.6460235
CAGTCAC258600.064.6226927
GCTATGA257700.064.30636636
CTCGTAT256100.064.2863744
TCACCGG258700.064.2633930
GAACTCC260650.064.2035521
TCCAGTC261150.064.0775225
TGAACTC261450.064.007120
CACACGT262550.063.98447812
GCACACG264750.063.46600311
CGGCTAT262050.063.34038534
Produced by FastQC (version 0.11.2)