FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458579
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2106274.724083614981365TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC246100.064.3528347
ACTCCAG247250.064.09885423
CACACGT250050.063.9072712
CCAGTCA248050.063.82085826
GCACACG250550.063.79298811
GAACTCC248800.063.755821
CTATGAT245800.063.7080937
CAGTCAC249100.063.701427
TCGTATG248500.063.6468145
AGTCACC248450.063.6278728
GCTATGA248700.063.28898236
TGAACTC251200.063.2302720
CTCCAGT250850.063.0673424
ACGTCTG253200.063.02998415
CCGGCTA251150.062.91120533
GGCTATG251050.062.8778435
CGTATGC252300.062.77142746
AGCACAC255650.062.57512710
ACACGTC255500.062.54408313
AACTCCA254950.062.32768222
Produced by FastQC (version 0.11.2)