FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301804
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1212383.6718714981264786TruSeq Adapter, Index 7 (97% over 38bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG146240.44290939135091No Hit
AAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAA81410.24656218237060712No Hit
GGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTG65710.1990124186656749No Hit
AAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAA54420.16481898986130006No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC52350.1585496898059364No Hit
TAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGTCCACTA49340.14943346122301626No Hit
GGCAATTTCAAAATCAGGGATAGGACCGTCCACTAGACAAGCGGCTCCGA48410.14661681916915723No Hit
GAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGCTCGATCG43700.13235189005767756No Hit
ACCGAATAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGT35110.10633580915160319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG138700.065.0753128
ACTCCAG142100.063.67182523
GTATGCC141250.063.5336547
GTCACGA142200.063.4243729
ACACGTC143600.063.2700513
GAACTCC143000.063.22214521
GCACACG143900.063.2101511
CAGTCAC143250.063.10607527
CTCCAGT143500.062.9530724
CATCTCG138350.062.87919641
TCGTATG142800.062.74504545
CACACGT144950.062.7049312
CACGTCT145150.062.52208314
TCCAGTC144400.062.4637525
CGAGATT144300.062.45568533
AGCACAC145750.062.35980210
CGTATGC144250.062.21138446
TGAACTC145750.062.1013120
CCAGTCA145900.061.8455526
GCCGTCT144350.061.58357251