Basic Statistics
Measure | Value |
---|---|
Filename | H3WGNAFXY_n01_79t1_2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3301804 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT | 121238 | 3.6718714981264786 | TruSeq Adapter, Index 7 (97% over 38bp) |
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG | 14624 | 0.44290939135091 | No Hit |
AAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAA | 8141 | 0.24656218237060712 | No Hit |
GGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTG | 6571 | 0.1990124186656749 | No Hit |
AAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAA | 5442 | 0.16481898986130006 | No Hit |
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC | 5235 | 0.1585496898059364 | No Hit |
TAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGTCCACTA | 4934 | 0.14943346122301626 | No Hit |
GGCAATTTCAAAATCAGGGATAGGACCGTCCACTAGACAAGCGGCTCCGA | 4841 | 0.14661681916915723 | No Hit |
GAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGCTCGATCG | 4370 | 0.13235189005767756 | No Hit |
ACCGAATAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGT | 3511 | 0.10633580915160319 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGTCACG | 13870 | 0.0 | 65.07531 | 28 |
ACTCCAG | 14210 | 0.0 | 63.671825 | 23 |
GTATGCC | 14125 | 0.0 | 63.53365 | 47 |
GTCACGA | 14220 | 0.0 | 63.42437 | 29 |
ACACGTC | 14360 | 0.0 | 63.27005 | 13 |
GAACTCC | 14300 | 0.0 | 63.222145 | 21 |
GCACACG | 14390 | 0.0 | 63.21015 | 11 |
CAGTCAC | 14325 | 0.0 | 63.106075 | 27 |
CTCCAGT | 14350 | 0.0 | 62.95307 | 24 |
CATCTCG | 13835 | 0.0 | 62.879196 | 41 |
TCGTATG | 14280 | 0.0 | 62.745045 | 45 |
CACACGT | 14495 | 0.0 | 62.70493 | 12 |
CACGTCT | 14515 | 0.0 | 62.522083 | 14 |
TCCAGTC | 14440 | 0.0 | 62.46375 | 25 |
CGAGATT | 14430 | 0.0 | 62.455685 | 33 |
AGCACAC | 14575 | 0.0 | 62.359802 | 10 |
CGTATGC | 14425 | 0.0 | 62.211384 | 46 |
TGAACTC | 14575 | 0.0 | 62.10131 | 20 |
CCAGTCA | 14590 | 0.0 | 61.84555 | 26 |
GCCGTCT | 14435 | 0.0 | 61.583572 | 51 |