FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956712
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1243203.1420027538016413TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT53630.13554183372456727TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA167000.064.6743728
CCAGTCA168300.063.90688326
GTATGCC142650.063.81479347
ACTCCAG169100.063.72873323
CGTATGC140950.063.4174246
CAGTCAC170850.063.05309727
TCGTATG139750.063.03535545
CACACGT173700.062.66330712
GCACACG173850.062.52950711
GAACTCC172700.062.50161721
TGAACTC174000.062.19556820
GTCACAT173500.062.19089529
CTCCAGT173800.061.90465524
AACTCCA174850.061.753122
TCCAGTC174500.061.67638425
CACATTC175000.061.66172831
AATCTCG148750.061.65579641
TCTGAAC177650.061.23138418
ACGTCTG178200.061.12095315
CGTCTGA179250.060.821516
Produced by FastQC (version 0.11.2)