FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t0_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t0_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4103057
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT287940.7017694367882289TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC37100.057.27544840
TATCTCG38450.055.53761341
GAACTCC41050.052.77979321
AGTCACC41400.052.24458328
GCTATCT40800.051.91101539
GTCACCT42050.051.4363729
GTATGCC41700.051.27996447
CACACGT43900.049.82623312
ACACGTC44200.049.40886713
CACGTCT44600.048.80879614
CCAGTCA44700.048.5483426
GAAGCTA44550.048.32720636
CTCGTAT45000.048.06384744
CTCCAGT45350.048.0068624
ACTCCAG45800.047.7650323
GCACACG45900.047.73140311
AGCTATC44700.047.69513738
TGAACTC45850.047.33008220
TCGTATG45750.047.12291745
CGTATGC46300.046.86549846
Produced by FastQC (version 0.11.2)