FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477288
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT519951.4952744782715726TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT138970.3996505322538714TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGAT81590.23463687793475835TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTAT75920.2183310671994957TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTAT66330.19075210336331072TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGAT55390.15929080363777748TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTTT43690.12564389259675932TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTTT41090.11816680125431084TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG183950.064.32905633
GAACTCC187550.063.7847421
AGTCACT187650.063.59970528
GTCACTC187750.063.43534529
GCACACG188950.063.33065811
ACTCCGC187650.063.09794632
AACTCCA190100.062.9107222
ACACGTC190750.062.86148513
CAGTCAC190250.062.8225227
ACTCCAG190400.062.7178823
CACACGT191250.062.6971412
ACGTCTG191200.062.64031215
CTCCAGT190850.062.5333224
TCCAGTC190800.062.45799625
CGCGAAA189500.062.31159236
CACTCCG190550.062.24696431
CACGTCT192500.062.2172914
TCACTCC191400.061.93395630
CCAGTCA192700.061.84216726
CCGCGAA191600.061.71999435
Produced by FastQC (version 0.11.2)