FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860492
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT115310.4031124715608364TruSeq Adapter, Index 1 (97% over 36bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG82930.289915161447751No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC35360.12361509838167699No Hit
GTGAACTACTGTGGAAGTTACTGACGTAAGATTACGCCGCGTGAATACCG34250.11973464704673183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG16350.052.66787735
TAGATCT16050.052.56182539
AGTCACA17100.051.1677628
CGATAGA16700.051.1448436
ACACGTC18550.047.73414613
CAGTCAC18500.047.4847927
GCACACG18550.047.356811
CACACGT18750.046.8516612
GAACTCC18900.046.48307421
CCAGTCA18850.046.42068526
ATAGATC18350.046.3552238
AGATCTC18350.046.1636540
CACGTCT19700.045.12529414
CTCCAGT19400.045.10463324
CGTATGC19800.043.8368246
GTCACAG20000.043.7484429
CACAGCG20100.043.707231
TCCAGTC20100.043.5338325
GCCGTCT20100.043.3566651
ACTCCAG20350.042.99901223
Produced by FastQC (version 0.11.2)