FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666807
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT4443799.522120799081685TruSeq Adapter, Index 27 (97% over 39bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG60400.12942467944356817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA484250.067.1148536
ACTCGAT480300.067.0552837
ATTACTC488550.066.7405534
ACATTAC490350.066.7189832
GTATGCC488700.066.6828447
TTACTCG489350.066.6013835
AGTCACA493500.066.4299828
CCAGTCA492550.066.3968126
ACTCCAG493750.066.28577423
CTCCAGT494250.066.147924
GAACTCC495500.066.1434921
TCGTATG492300.066.13122645
CGTATGC492750.066.1205546
TCGATCT480800.066.0608639
CATTACT495050.066.00777433
CAGTCAC496300.066.0058427
CACACGT500300.065.93281612
GCACACG500800.065.9291811
CACATTA497150.065.89088431
TCTCGTA484950.065.8358543
Produced by FastQC (version 0.11.2)