FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_79t-1_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79t-1_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096000
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2765346.7513183593749995TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT298300.066.3159637
TACTCGA301000.066.301836
GTATGCC305300.065.7113847
TTACTCG305500.065.49705535
ATTACTC306650.065.34778634
ACATTAC308600.065.1804132
AGTCACA309900.065.15227528
GAACTCC310400.065.043221
CCAGTCA310150.065.039226
TCGTATG309000.064.87845645
TCTCGTA303500.064.8433343
TCGATCT302200.064.7882839
CATTACT310500.064.6688433
CTCGTAT306650.064.6672344
GTCACAT311950.064.6568129
TGAACTC312650.064.6534820
CAGTCAC312350.064.5964227
CGTATGC311550.064.4597646
CTCCAGT313350.064.3973524
CTCGATC304600.064.3237738
Produced by FastQC (version 0.11.2)